Techniques
Getting started with Immunohistochemistry
Immunohistochemistry isn’t just a useful clinical tool, it also has great applications as a basic research tool. We’ll walk you through the immunohistochemistry basics to get you off to a flying start.
Read More Automating IHC and RNAscope® ISH for academic research: Insights from equine arteritis virus and COVID-19
Automating IHC and RNAscope® ISH for academic research: Insights from equine arteritis virus and COVID-19 Date: Thursday August 26th 2021Time: 8:30am Delhi, 10am Jakarta, 11am Singapore, 1pm Sydney Speaker Dr. Mariano Carossino DVM, PhD, Dipl. ACVM Assistant Professor (appointed) Department of Pathobiological Sciences & Louisiana Animal Disease Diagnostic LaboratoryLouisiana State University, School of Veterinary Medicine Dr.…
Read More That Other Number – The Meaning Of Numerical Aperture In Microscopy
Do you know what that NA number is on your objective? We walk you through what the numerical aperture is and why it’s important.
Read More An Objective View: What do These Abbreviations Mean on Microscope Objectives?
There are a large number of microscope objective abbreviations relating to optical aberrations; here we’ll shed some light on some of the most common ones to get you up to speed in no time!
Read More Improving the Expression of Insoluble and Inactive Proteins Using Osmotic Shock
Discover a better way to improve the expression of your insoluble and inactive proteins using osmotic shock.
Read More Introduction to Yeast Media: How to Feed Your Fungus
Are you finding the variety of available yeast media overwhelming? Read our guide to understand what’s in each and which is right for your fungus.
Read More Cell Counting with a Hemocytometer: As easy as 1, 2, 3…
Do hemocytometers look scary and complicated with their multiple tiny squares, boxes, and grids? Take a look at our article and see how easy it actually is to use a hemocytometer.
Read More Live Imaging of Phagocytosis: Your Simple Guide to Capturing the Action
Live imaging of phagocytosis helps capture the details of this dynamic process. Discover tips and tricks to visualizing this important cellular process.
Read More Mass Spectrometry in Biological Research – A Guide for Beginners
Mass spectrometry can feel intimidating. Read this easy-to-follow guide to demystify mass spectrometry and learn how it can help your research.
Read More See The Hidden: Artificial Intelligence in Microscopy
See The HiddenArtificial Intelligence in Microscopy Join the Leica Microsystems Virtual Workshop Introduction to AI for Microscopists: all you need to know Machine and deep learning technologies already play a major role in everyday life – think Alexa, Tesla’s Autopilot, Spotify, Amazon or YouTube. In the last 3 years, microscopists have started to use “AI…
Read More Working with Membrane Proteins: Tips for Expressing, Extracting, and Purifying Your Membrane Protein Sample
Working with membrane proteins can be tricky. Read on to get tried-and-tested tips and tricks for expressing, extracting, and purifying these water-phobic proteins.
Read More Surviving the Big Chill: Freezing and Thawing Mammalian Cells in the Lab
Cryopreservation is crucial to the long-term maintenance of cells, so it’s important that you’re clued up on your freeze–thaw cycles. Check out our top tips for freezing and thawing cells.
Read More A Beginner’s Guide to The Point Spread Function
Learn how the Point Spread Function affects what you see through your microscope and discover what you can do to improve your images.
Read More Intravital Microscopy of Cancer
Intravital Microscopy of Cancer Available on Demand In this webinar, you will learn: about intravital microscopy techniques that allow you to monitor stem cells and cancer cells in real-time in vivo; how these techniques can help you study the identity and migratory behavior of cancer stem cells; how intravital microscopy uses multiphoton confocal microscopy to…
Read More Lab Basics: How Alkaline Lysis Works
Alkaline lysis for plasmid isolation? That’s like the ABCs in a molecular biology lab. Read this detailed article to understand the process behind this common technique.
Read More A Complete Guide to How Nucleic Acid Extraction Kits Work
Nucleic acid extraction kits are routine in today’s molecular biology labs. Read on to know about what is inside these black boxes of wonder and how you can get the best results for your preps!
Read More Quick and Easy Sequencing of SARS-CoV-2 to Identify Variants of Concern
Quick and Easy Sequencing of SARS-CoV-2 to Identify Variants of Concern Available On Demand Speaker Jordan RoseFigura, PhD Director of Material SciencesSwift Biosciences Dr. Jordan RoseFigura completed her Ph.D. in Chemistry at UC Berkeley where she studied the mechanism of PQQ formation. She then took up an NIH Postdoctoral Fellowship at The Rockefeller University where…
Read More Multiplex CRISPR Gene Editing: What You Need to Know
If you need a multi-gene knockout or large-scale genomic modification, or want reduced off-target effects, then multiplex CRISPR is for you!
Read More Unnatural Amino Acids: Essential Tools in Synthetic Biology and Your Research
Discover what unnatural amino acids are, their applications, and how they can be used in your research in our beginner’s guide.
Read More Controlling Gene Expression with CRISPR Interference (CRISPRi)
CRISPR interference allows for the regulation of gene expression in vivo. Here’s a short guide to how it works.
Read More Advances to SYNTHIA™ Retrosynthesis Software and How They Have Accelerated Chemical Development at GlaxoSmithKline (GSK)
Advances to SYNTHIA™ Retrosynthesis Software and How They Have Accelerated Chemical Development at GlaxoSmithKline (GSK) Available On Demand John Jin Lim, MS Senior ScientistAPI ChemistryGlaxoSmithKline plc (GSK) John Lim (MS) leads the CASD Team at GSK Chemical Development, which uses computational tools, such as SYNTHIA™ retrosynthesis software, for the route selection process. He obtained his…
Read More Choosing The Right Blood Collection Tubes
Selecting the right blood collection tubes for your experiment is cruical if you don’t want to fall at the first hurdle. What tubes do you use for which type of blood sample? Read on to find out more.
Read More Optimal Conditions for Live-Cell Imaging
Live-cell imaging can bring a lot of clarity to cellular processes, but keeping your cells happy can be tricky. Read on to learn about 4 key parameters for achieving optimal conditions for live-cell imaging.
Read More An Introduction to Live Cell Imaging
Read on to learn more about live cell imaging, including how high rate microscopy can help you capture rapid cellular processes.
Read More What is PCR? – The Beginner’s Guide
If you need to copy, sequence, or quantify DNA, you need to know PCR. But what is PCR? Read our guide to the PCR process, and discover tips to help you avoid the most common PCR pitfalls.
Read More Visualising protein degradation and aggregation in the living cell
Visualising protein degradation and aggregation in the living cell Available On Demandat MicroscopyFocus.com Watch Now In this webinar, Prof. Eric Reits will discuss how several neurodegenerative disorders, including Alzheimer’s, Parkinson’s and Huntington’s disease, are characterized by the formation of protein aggregates, and that components of the ubiquitin-proteasome system (UPS) co-localize in these aggregates. This has…
Read More Life beyond the pixels: deep learning methods for single cell analysis
Life beyond the pixels:Deep learning methods for single cell analysis Available On Demand In this webinar, you will discover: the computational steps in the analysis of single cell-based large-scale microscopy experiments; a novel microscopic image correction method designed to eliminate illumination and uneven background effects; new single-cell image segmentation methods; the Advanced Cell Classifier, a…
Read More A Step-by-Step Guide to Designing qPCR Primers
qPCR primer design is a bit of science, a bit of magic, and a little bit of luck. Here’s the science to help you design the best primers for your experiments.
Read More How to Identify Supercoils, Nicks and Circles in DNA Plasmid Preps
Why do you get three bands when running uncut plasmid DNA on agarose gels. Discover the answer and how it can help improve your DNA plasmid preps.
Read More Which Type of Ethanol Should I Use?
Want to know more about ethanol grades commonly used in the lab? We help you make sense of your flammables cabinet with our rundown of the ethanol grades typically used in molecular biology, as well as some important rules for how to use them correctly.
Read More CRISPR-Cas9 Genome Editing: Weighing the Pros and Cons
Constant Current or Voltage in SDS-PAGE: The Great Debate
It’s not always easy deciding whether to run electrophoresis at a constant voltage, current, or power. Here, we outline the differences to help you make an informed decision.
Read More How History Shaped Modern Optical Microscopes, Part Two: Corrected Lenses and Objectives
Discover how chromatic and geometric imaging aberrations have been corrected over the last few centuries with the development of corrected lenses and objectives.
Read More 5 Ways to Clean Up A DNA Sample
Are you struggling with your DNA clean-up? Then check out our top five methods so you can pick the best option for your experiments.
Read More See The Hidden: Virtual Neurosciences Workshop
See The Hidden: Virtual Neurosciences Workshop This live online event brings together a series of scientific talks and microscopy showcases, centering around four specific topics in microscopy – 3D real time imaging*, laser microdissection, confocal nanoscopy and high pressure freezing techniques in electron microscopy. Each section will kick off with a scientific talk from one…
Read More How History Shaped Modern Optical Microscopes, Part One: Simple and Compound Microscopes
Discover the history of simple and compound microscopes in this first of our two-part series on the history of microscopes.
Read More Ethanol Precipitation of DNA and RNA: How it Works
Using ethanol precipitation to isolate or concentrate your nucleic acids? Find out how this routinely used technique works, and get tips to produce the best results.
Read More Screening in the Modern CRISPR Era
Screening in the Modern CRISPR Era Available On Demand Speaker Andrew Ravanelli Principal Scientist, Genome and Epigenome EditingMerck KGaA, Darmstadt, Germany Andrew obtained his Ph.D. in Cell Biology from Duke University, where he used mouse and zebrafish models to dissect cellular mechanisms of early tissue formation. He completed a postdoctoral fellowship in Pediatrics at the…
Read More 4 Easy Steps to Analyze Your qPCR Data Using Double Delta Ct Analysis
New to qPCR? Here’s a quick summary of one of the two most common analysis methods – double delta Ct analysis.
Read More Advanced Sectioning Techniques: How to Section Difficult Tissues
Are you having problems with tissue sectioning? Follow these 10 tissue sectioning tips to create the perfect tissue section every time without stressing out.
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